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mouse anti dsdna  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti dsdna
    Mouse Anti Dsdna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+dsdna/ds+DNA+Marker+Antibody/10__1007_slash_s00018___026___06458___w-325-30-34
    Average 95 stars, based on 125 article reviews
    mouse anti dsdna - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Staining:

    Article Title: X-ray preactivated reversible persistent luminescence enables photodynamic immunotherapy of deep tumors.
    Article Snippet: .. 4 h after treatment, the cells were stained with anti-dsDNA (Santa Cruz Biotechnology, sc-58749) primary antibody and FITC secondary antibody (Proteintech, SA00013-2), and visualized using a confocal laser scanning microscope (Zeiss LSM 880, Germany). ..

    Laser-Scanning Microscopy:

    Article Title: X-ray preactivated reversible persistent luminescence enables photodynamic immunotherapy of deep tumors.
    Article Snippet: .. 4 h after treatment, the cells were stained with anti-dsDNA (Santa Cruz Biotechnology, sc-58749) primary antibody and FITC secondary antibody (Proteintech, SA00013-2), and visualized using a confocal laser scanning microscope (Zeiss LSM 880, Germany). ..

    Selection:

    Article Title: Exploring the neuroprotective role of artesunate in mouse models of anti-NMDAR encephalitis: insights from molecular mechanisms and transmission electron microscopy
    Article Snippet: .. In this study, we utilized a selection of antibodies, including anti-PTEN-induced putative kinase 1 (PINK1) (23274–1-AP), anti-caspase3 (19677–1-AP), anti-p62 (18420–1-AP), and anti-GAPDH (10494–1-AP) obtained from Proteintech (Wuhan, China); anti-cleaved caspase-3 (9661 T) sourced from Cell Signaling Technology Inc. (Massachusetts, USA); anti-LC3B (NB100-2220) from Novus; anti-Parkin (sc-32282) and anti-dsDNA (sc-58749) from Santa Cruz Biotechnology, Inc. (Texas, USA); anti-GluN1 procured from Synaptic Systems GmbH (Göttingen, Germany); anti-MAP2 (M4403) from Sigma-Aldrich (St. Louis, MO, USA); and anti-β-actin from ABclonal (Wuhan, China). .. Additional materials included MitoSOX Red (#M36008), MitoTracker (M22426), tetramethylrhodamine (TMRM, I34361), and the Melon Gel IgG Spin Purification Kit (45,206) from Thermo Fisher Scientific, Inc. (Waltham, MA, USA); the B-27 Plus Neuronal Culture System (A3653401) from Gibco (Carlsbad, CA, USA) from Beyotime (Shanghai, China).

    Article Title: Exploring the neuroprotective role of artesunate in mouse models of anti-NMDAR encephalitis: insights from molecular mechanisms and transmission electron microscopy.
    Article Snippet: .. In this study, we utilized a selection of antibodies, including anti-PTEN-induced putative kinase 1 (PINK1) (23274–1-AP), anti-caspase3 (19677–1-AP), anti-p62 (18420–1-AP), and anti-GAPDH (10494–1-AP) obtained from Proteintech (Wuhan, China); anti-cleaved caspase-3 (9661 T) sourced from Cell Signaling Technology Inc. (Massachusetts, USA); anti-LC3B (NB100-2220) from Novus; anti-Parkin (sc-32282) and anti-dsDNA (sc-58749) from Santa Cruz Biotechnology, Inc. (Texas, USA); anti-GluN1 procured from Synaptic Systems GmbH (Göttingen, Germany); anti-MAP2 (M4403) from Sigma-Aldrich (St. Louis, MO, USA); and anti-βactin from ABclonal (Wuhan, China). .. Additional materials included MitoSOX Red (#M36008), MitoTracker (M22426), tetramethylrhodamine (TMRM, I34361), and the Melon Gel IgG Spin Purification Kit (45,206) from Thermo Fisher Scientific, Inc. (Waltham, MA, USA); the B-27 Plus Neuronal Culture System (A3653401) from Gibco (Carlsbad, CA, USA) from Beyotime (Shanghai, China).

    Incubation:

    Article Title: Trilaciclib triggers a neutrophil-related immune response and sensitizes non-small cell lung cancer to anti-PD-1 therapy
    Article Snippet: .. Cells were permeabilized with 0.2% Triton X-100 for 10 min at room temperature and blocked with 5% normal goat serum for 1 h. Samples were incubated with primary antibodies: Anti-Lamin B1(1:100, Santa Cruz, sc-377000), Anti-cGAS (1:100, Proteintech, 26416-1-AP), Anti-dsDNA (1:200, Santa Cruz, sc-58749) at 4°C overnight. ..



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    ( A, B ) DDX21 immunofluorescence showing that treatment with 0.5 μM BPA for 72 hours increases DDX21 foci formation in in RPE1 ( A ) and HeLa ( B ) cells. At least 105 cells were quantified for each condition. The mean value is represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). Representative micrographs, with scale bars representing 10 and 20 µm are shown. ( C ) Western blot showing that HeLa and RPE1 cells do not express the estrogen receptor (ER). ER-expressing MCF7 cells used as a positive control. ( D ) Proximity ligation assay showing that treatment with 0.5 μM BPA for 72 hours or 0.5 μM Cisplatin for 24 hours increases <t>the</t> <t>S9.6-dsDNA</t> co-localization in HeLa cells. At least 101 cells were quantified for each condition. The mean value is represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). ( E ) Representative PLA experiment showing an increase in the number of S9.6-dsDNA foci in sgDDX21 cells, as compared to control HeLa cells, after 0.5 μM BPA for 72 hours. The mean values are represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). Representative micrographs, with scale bars representing 10 µm are shown. ( F ) The means of 3 independent S9.6-dsDNA PLA experiments showing an increase in the mean number of foci in sgDDX21 cells, normalized to control HeLa cells, after 0.5 μM BPA for 72 hours across three independent replicates. The mean values are represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired).
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    ( A, B ) DDX21 immunofluorescence showing that treatment with 0.5 μM BPA for 72 hours increases DDX21 foci formation in in RPE1 ( A ) and HeLa ( B ) cells. At least 105 cells were quantified for each condition. The mean value is represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). Representative micrographs, with scale bars representing 10 and 20 µm are shown. ( C ) Western blot showing that HeLa and RPE1 cells do not express the estrogen receptor (ER). ER-expressing MCF7 cells used as a positive control. ( D ) Proximity ligation assay showing that treatment with 0.5 μM BPA for 72 hours or 0.5 μM Cisplatin for 24 hours increases <t>the</t> <t>S9.6-dsDNA</t> co-localization in HeLa cells. At least 101 cells were quantified for each condition. The mean value is represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). ( E ) Representative PLA experiment showing an increase in the number of S9.6-dsDNA foci in sgDDX21 cells, as compared to control HeLa cells, after 0.5 μM BPA for 72 hours. The mean values are represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). Representative micrographs, with scale bars representing 10 µm are shown. ( F ) The means of 3 independent S9.6-dsDNA PLA experiments showing an increase in the mean number of foci in sgDDX21 cells, normalized to control HeLa cells, after 0.5 μM BPA for 72 hours across three independent replicates. The mean values are represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired).
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    ( A, B ) DDX21 immunofluorescence showing that treatment with 0.5 μM BPA for 72 hours increases DDX21 foci formation in in RPE1 ( A ) and HeLa ( B ) cells. At least 105 cells were quantified for each condition. The mean value is represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). Representative micrographs, with scale bars representing 10 and 20 µm are shown. ( C ) Western blot showing that HeLa and RPE1 cells do not express the estrogen receptor (ER). ER-expressing MCF7 cells used as a positive control. ( D ) Proximity ligation assay showing that treatment with 0.5 μM BPA for 72 hours or 0.5 μM Cisplatin for 24 hours increases <t>the</t> <t>S9.6-dsDNA</t> co-localization in HeLa cells. At least 101 cells were quantified for each condition. The mean value is represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). ( E ) Representative PLA experiment showing an increase in the number of S9.6-dsDNA foci in sgDDX21 cells, as compared to control HeLa cells, after 0.5 μM BPA for 72 hours. The mean values are represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). Representative micrographs, with scale bars representing 10 µm are shown. ( F ) The means of 3 independent S9.6-dsDNA PLA experiments showing an increase in the mean number of foci in sgDDX21 cells, normalized to control HeLa cells, after 0.5 μM BPA for 72 hours across three independent replicates. The mean values are represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired).
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    Image Search Results


    ( A, B ) DDX21 immunofluorescence showing that treatment with 0.5 μM BPA for 72 hours increases DDX21 foci formation in in RPE1 ( A ) and HeLa ( B ) cells. At least 105 cells were quantified for each condition. The mean value is represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). Representative micrographs, with scale bars representing 10 and 20 µm are shown. ( C ) Western blot showing that HeLa and RPE1 cells do not express the estrogen receptor (ER). ER-expressing MCF7 cells used as a positive control. ( D ) Proximity ligation assay showing that treatment with 0.5 μM BPA for 72 hours or 0.5 μM Cisplatin for 24 hours increases the S9.6-dsDNA co-localization in HeLa cells. At least 101 cells were quantified for each condition. The mean value is represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). ( E ) Representative PLA experiment showing an increase in the number of S9.6-dsDNA foci in sgDDX21 cells, as compared to control HeLa cells, after 0.5 μM BPA for 72 hours. The mean values are represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). Representative micrographs, with scale bars representing 10 µm are shown. ( F ) The means of 3 independent S9.6-dsDNA PLA experiments showing an increase in the mean number of foci in sgDDX21 cells, normalized to control HeLa cells, after 0.5 μM BPA for 72 hours across three independent replicates. The mean values are represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired).

    Journal: bioRxiv

    Article Title: Genome-wide CRISPR screens identify DNA repair and R-loop suppression as regulators of the cellular sensitivity to environmentally relevant Bisphenol A exposure

    doi: 10.64898/2026.04.13.718249

    Figure Lengend Snippet: ( A, B ) DDX21 immunofluorescence showing that treatment with 0.5 μM BPA for 72 hours increases DDX21 foci formation in in RPE1 ( A ) and HeLa ( B ) cells. At least 105 cells were quantified for each condition. The mean value is represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). Representative micrographs, with scale bars representing 10 and 20 µm are shown. ( C ) Western blot showing that HeLa and RPE1 cells do not express the estrogen receptor (ER). ER-expressing MCF7 cells used as a positive control. ( D ) Proximity ligation assay showing that treatment with 0.5 μM BPA for 72 hours or 0.5 μM Cisplatin for 24 hours increases the S9.6-dsDNA co-localization in HeLa cells. At least 101 cells were quantified for each condition. The mean value is represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). ( E ) Representative PLA experiment showing an increase in the number of S9.6-dsDNA foci in sgDDX21 cells, as compared to control HeLa cells, after 0.5 μM BPA for 72 hours. The mean values are represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). Representative micrographs, with scale bars representing 10 µm are shown. ( F ) The means of 3 independent S9.6-dsDNA PLA experiments showing an increase in the mean number of foci in sgDDX21 cells, normalized to control HeLa cells, after 0.5 μM BPA for 72 hours across three independent replicates. The mean values are represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired).

    Article Snippet: Antibodies used were: S9.6 (Kerafast ENH001), dsDNA (Novus Biologicals NBP3-07302).

    Techniques: Immunofluorescence, Two Tailed Test, Western Blot, Expressing, Positive Control, Proximity Ligation Assay, Control